| Sea Cucumber extract for fighting eimeriosis |
| Paper ID : 1135-ISCHU |
| Authors |
|
Yossef Ahmed Mohamed El-Sayed * Helwan University |
| Abstract |
| Coccidiosis is one of the most important protozoan infections that affect farm and domestic animals. A typical anticoccidial medicine is Amprolium, but the emergence of drug-resistant strains of Eimeria necessitates the creation of novel therapies. The current study aimed to determine the anthelmintic, anticoccidial activity of Holothuria poli extract (HpE) compared to the drug Amprolium in mice infected with Eimeria papillata. Also, to estimate the protective role of HpE on E. papillata-induced jejunal damage in mice. A total of 25 Swiss albino male mice were divided into five equal groups. The first group served as a control group gavaged daily with distilled water. The second group was treated daily with HpE (200 mg/kg), and group 3 was infected with 1000 E. papillata oocysts. Group 4 was infected and treated with HpE while the last group was infected and treated with Amprolium (120 mg/kg). Results indicated that infrared spectroscopy of HpE showed the presence of several expected active classes of phytochemical compounds. HpE was effective in reducing the number of E. papillata oocysts found in mice feces as well as the infection-caused jejunal histological damage. Also, the jejunal tissues of infected-treated mice showed a considerable reduction in the intracellular developmental Eimeria stages. Moreover, the extract was able to regulate the oxidative status in the infected jejunum which was evidenced through the determination of GSH, NO, and MDA concentration as well as the activity of CAT and SOD in the jejunum of mice infected with E. papillata. In our study, we aspire to apply safe therapeutic methods against murine eimeriosis. Eimeria papillata is a fascinating and challenging parasite that has significant implications for research, animal welfare, and public health so further studies are required to know the mechanism of HpE action. |
| Keywords |
| Eimeriosis, mice, Sea cucumber extract, Jejunum |
| Status: Abstract Accepted (Poster Presentation) |