| ANTICANCER EFFECT OF ACTINOMYCETES SECONDARY METABOLITE AGAINST BREAST CANCER CELL LINE (MCF-7); CYTOLOGICAL AND MOLECULAR STUDIES |
| Paper ID : 1132-ISCHU |
| Authors |
|
Asmaa Salah Ahmed *1, Basma Nasr Hassan2, Ahmed Saed Alazzouni3, Sarah Mohamed Abdo4 1Teaching assistant of Histology and Histochemistry, Zoology and Entomology Department, Faculty of Science, Helwan University. 2Assistant professor of Histology and Histochemistry Department of Zoology and Entomology Faculty of science-Helwan University 3Professor of Histology and Histochemistry Department of Zoology and Entomology Faculty of science-Helwan University 4Assist. Prof. of Biochemistry Department of Chemistry Faculty of Science-Helwan University |
| Abstract |
| Heliomycin is a natural compound extracted from the metabolites of Actinomycetes bacteria. We aim to evaluate the anticancer role of Heliomycin, against Tamoxifen as a standard drug. Flow cytometry and analysis of the cell cycle to detect the ratios of apoptosis, and cell cycle arrest were performed, and the genes expression was assayed using real-time RT-PCR. CK-19 protein was assessed using immunocytochemistry. Also, cytopathological alterations were evaluated using H&E stain and electron microscope examination. qRT- PCR showed that HER-2 expression was less elevated in tamoxifen-treated cells than in heliomycin-treated cells in the control cell. For P53 it showed significant up-regulation in heliomycin-treated cells than tamoxifen-treated cells compared to the control. In contrast, ERα, TNFα, and TLR-4 showed the reverse. The flow cytometry assay revealed a considerable increase in early and late apoptosis in the treated group with Tamox. and Helio. compared with the control. H&E stain and ultrastructure study showed cell apoptosis and chromatin degradation and cell shrinkage in treated cells against control. Immunohistochemically, CK-19 showed a severe reaction in control cells while a mild reaction in heliomycin-treated cells versus a moderate reaction in tamoxifen-treated cells. Heliomycin possesses accepted anticancer behaviour against breast cancer MCF-7 cells, as it activates cancerous cell apoptosis with upregulation of tumour suppressor genes. |
| Keywords |
| Breast cancer, Cell culture, Heliomycin, Chemotherapy, Tumour supressor gene. |
| Status: Abstract Accepted (Poster Presentation) |