| Isolation and Biological activity of Aspergillus fumigatus from Damietta Governorate in Egypt. |
| Paper ID : 1127-ISCHU |
| Authors |
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Wesam Bakry * Helwan university faculty of science botany and microbiology department |
| Abstract |
| The marine environments support a vast spectrum of biologically active animals. The purpose of the current research was to determine whether a fungal species isolated from the Egyptian governorate of Damietta possessed antibiotic activity. After being isolated, purified, and genetically characterised, the isolate was identified as Aspergillus fumigatus with accession number 26. The antimicrobial screening of extracts with ethyl acetate compounds, extracts with petroleum ether compounds, extracts derived with methanol, and chloroform extracts with Aspergillus fumigatus against several pathogenic microbes was performed using the well diffusion method, and the highest inhibitions were 39mm against Staphylococcus aureus, 36mm against Enterobacter cloacae, and 35mm against Salmonella. Except for Streptococcus mutants and Bacillus subtilis, ethyl acetate extract had modest antibacterial effectiveness with inhibition of 7mm and strong inhibition of 15mm and 13mm with petroleum ether, respectively. In comparison to petroleum ether extracts, ethylacetate extracts had the highest percent DPPH scavenging activity of 94.22 percent at a concentration of 3200 ug/ml, with an IC50 of 150 ug/ml. At a concentration of 500 ug/ml, the cytotoxic activity of ethyl acetate extract was reported to be 26.6 1.1 ug/ml for toxic cells of the human colon, 12.7 0.8 ug/ml for toxic cells of the human breast, and 50.4 2.4 ug/ml for hazardous cells of the human hepatocells. The main three components were diethyl phthalate, tributylacetyl-citrate, 1-propene-1,2,3-tricarboxylic acid, and tributyl ester. In comparison to petroleum ether extracts, the ethyl acetate extract showed the highest % DPPH scavenging action, according to our data. The active ethyl acetate extract was chromatographically separated using GCMS to determine the active principles responsible for the activities. |
| Keywords |
| Damietta governorate, antimicrobial activity, DPPH scavenging activity, Cytotoxic activity. |
| Status: Abstract Accepted (Poster Presentation) |