| Studying the protective role of IL-28B and its related miRNA in albino mice exposed to cigarette smoke |
| Paper ID : 1105-ISCHU |
| Authors |
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Basma Ashraf Ghanem1, Soad Nady Hamed *2, Doaa Ezz-Eldin Soliman3, Manal Mahmoud Hussein4 1Faulty of Science Helwan University 2Helwan University, Faculty of science, Zoology depart., Ain Helwan 3Helwan University, Faculty of science, Zoology depart., 4Helwan University, Faculty of science, Zoology depart. |
| Abstract |
| Cigarette smoke (CS) is one of the principal causes of inflammatory pulmonary diseases. The current study aimed to investigate the therapeutic effect of rhIL-28B on CS-induced inflammation. Animals were sub-acutely and sub-chronically exposed to research CS; 3cigarettes/time for 10 and 20 consecutive days, respectively. Then, mice were simultaneously and post treated with rhIL-28B (500ng/kg/b.w, IP). A significant increase in the total leukocytes' counts (TLC), IL-17 and IL-28B plasma levels as well as let7a expression was observed in all CS-exposed animals as compared to control ones. After simultaneous treatment, rhIL-28B injection repressed pulmonary inflammation by decreasing TLC and IL-17 plasma level while increasing IL-28B level in sub-acute and sub-chronic CS-exposed animals compared to untreated CS-exposed mice. Furthermore, at molecular level, let7a expression was kept upregulated with simultaneous rhIL-28B treatment. However, post treatment with rhIL-28B did not record a significant improvement. The current results revealed that rhIL-28B might be an effective therapy in alleviating CS-mediated lung inflammation by inducing Let7a expression and decreasing IL-17 levels. |
| Keywords |
| Cigarette smoke, lung inflammation, Let7a, IL-17, IL-28B, |
| Status: Abstract Accepted (Poster Presentation) |