Liver response during sepsis and after treatment with green nanosilver

Paper ID : 1092-ISCHU

Authors

Amal Marzouk Sayed Marey * Helwan University

Abstract

Sepsis is a potentially lethal infection-related disease induced by dysregulated host immunological responses. The treatment of sepsis is a substantial cost burden on healthcare systems all around the world. The study's purpose was to investigate the antioxidant and anti-inflammatory properties of pomegranate peel extract-derived nanosilver (PENPs) against sepsis-induced liver damage in rats. The current study investigated the effect of PENPs on liver injury in a male rat model of sepsis caused by cecal ligation and puncture. PENPs were identified as spherical particles with a diameter of 15±5 nm. PENPs contained phenolic and flavonoid compounds. After sepsis induction, the rats were given 20 mg/kg PENPs intraperitoneally every day for seven days. The oxidative condition of the liver was assessed by measuring the concentrations of reduced glutathione, nitric oxide, lipid peroxidation, and superoxide dismutase in the liver homogenate. Furthermore, the enzyme levels of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase were measured. Furthermore, we looked at histological alterations, immunohistochemistry expression of nuclear factor-B, and mRNA levels of IL1, IL6, TNF-, Bax, BCl2, and Casp-3. P-AgNPs were able to control gene expression affected by sepsis induction, as well as improve liver function and oxidative state. Based on our findings, we may conclude that PENPs have antioxidant action and may be effective in avoiding Sepsis-induced liver inflammation and damage.

Keywords

Sepsis, Liver, pomegranate, nanosilver, Oxidative stress, Inflammation, cytokines, Apoptosis, Rats

Status: Abstract Accepted (Oral Presentation)