| Next generation sequencing of embryos for testing the efficacy of advanced sperm selection techniques in males with high teratozoospermia index |
| Paper ID : 1087-ISCHU |
| Authors |
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Mohamed Nasreldin Abdelmagied *1, Maiada Moustafa Salama2, Hanan Ramadan Hamad3, Mohamed Abas Eid4 1B.Sc. of Biotechnology, Faculty of Science, Helwan University and M.Sc. student of Cell Biology, Histology and Genetics, Cairo University. 2Associate Professor of Embryology, Zoology & Entomology Department, Faculty of Science, Helwan University 3Professor of Genetics and Genomics, Zoology Department Faculty of science Cairo University 4Professor of Andrology, Faculty of Medicine – Cairo University |
| Abstract |
| Infertility affects about 10–15% of couples worldwide and 30–50% of these cases show a paternal cause of infertility which may badly affect the reproductive outcomes. One of the important assisted reproductive technologies (ART) is intracytoplasmic sperm injection (ICSI). It is characterized by its ability to accomplish fertilization regardless of the characteristics of the semen or the presence of defective spermatozoa. This therapy is regarded as the most effective way to treat infertility caused by the male factor. Teratozoospermic index (TZI) is a unique expression of sperm morphological assessment that has been suggested by WHO (1999) (The normal TZI is <1.6. If irrespective of the count and motility TZI is >1.8). Sperm DNA fragmentation (SDF) is suggested to be a major factor of male infertility that negatively affects fertilization and blastocyst developmental rate. The choice of high-quality sperm is expected to improve outcomes. Advanced sperm selection techniques employ sophisticated procedures to pick sperm that is mature, healthy, and structurally normal for fertilization. In this study, a total number of 264 couples were included, as 111 males have TZI <1.61 and were considered as the control group, and 153 males have TZI >1.81. This group was divided into two subgroups, the first one who underwent sperm selection (SS-group), (90 males) or not (NO-SS group), (63 males). In the NO-SS and SS-groups, the TZI was significantly (P<0.000) elevated as compared to the control group. Some of the females of each group are younger than 35 years old, and some are older than 35 years old. Although the euploidy, and aneuploidy rates, as well as low and high mosaic, were similar among the different groups. The percentage of sperm DNA fragmentation in the males of SS-group was significantly higher than in the controls and NO-SS group. The fertilization and blastocyst development rates are significantly higher in SS-group as compared to NO-SS group. In general, Sperm selection techniques are efficient techniques in increasing fertilization and blastocyst development rates in males with high teratozoospermia index. |
| Keywords |
| Teratozoospermia index, Sperm selection, ICSI, Preimplantation genetics testing for aneuploidy |
| Status: Abstract Accepted (Oral Presentation) |