Influence of Induced hyperprolactinemia on the histological structure and clusters of differentiations (CD4&CD8) of male mice spleen

Paper ID : 1072-ISCHU

Authors

Amira Amen * Ministry of labor

Abstract

Abstract: Prolactin (PRL) is a peptide hormone and is one of several hormones that are produced by the pituitary gland .Although PRL has traditionally been associated with lactation, recent evidence implicates PRL in more than 300 physiological functions corresponding to the wide distribution of prolactin receptors (PRLRs), including the reproduction, growth, osmoregulation, metabolism and immune regulation. The present study aimed to demonstrate the histological structure and immunohistochemical (IHC) alterations in the spleen of hyperprolactinemia (HPRL) of adult male mice (Mus musculus) by using metoclopramide (MCP) for different durations. The mice were divided into five groups; group I: control mice injected i.p. with saline solution for 10 weeks, groups II, III, IV and V: mice groups treated i.p. with MCP in a daily dose of 2.2 mg/kg/ b.w for different durations 2, 4, 7 and 10 weeks, respectively. A highly significant increase in the level of prolactin hormone and gradual significant increase in the body weight of mice groups II, III, IV and V was recorded. HPRL mice group II for 2 weeks showed obvious changes noticed in both red and white pulps. A large vacuolated areas with some lymphocytes were appeared all over the sections. Pyknotic picture was detected in red pulps as well as in PALS and in marginal zone which also became large with low intense of cellularity and also many giant cells were detected. HPRL mice group III for 4 weeks illustrated clear marked sever histological alteration in both red and white pulps. In which, many large vacuolated areas of lesion with some lymphocytes were appeared all over the sections. In addition, there was significant increase in macrophages, pyknotic nuclei, vesicular nuclei, giant cells, cell fragments in all splenic sections and some plasma cells. Moreover, The white pulp was detected with large dilated blood artery and vein which appeared with thick hyaline wall. While HPRL mice groups IV and V for 7 and 10 weeks illustrated marked histological changes in compared with spleen of control mice group I and their change were a time dependent. IHC study expressed intense immunoreactivity to CD4+/ CD8+ T-cells in the spleen tissue of control group (I), while in HPRL mice groups (III) CD4+ T-cells immunostain were markedly reduced and were reduced in other HPRL mice groups. CD8+ immunostaining was marked decreased in HPRL mice groups (III &V) &decreased in HPRL mice groups (II & IV). Image analysis of the mean percentage areas of CD4+ / CD8+ T-cells immunostain confirm the IHC study. In conclusion, the present study revealed that HPRL lead to histological alteration and immunohistochmical changes represented by significant decrease in CD4+/ CD8+ T-cell in spleen and such change were a time –dependent and finally led to immune senescence in spleen and subsequently weight gain. Therefore, MCP must be used under medical supervision and must be used with caution as a therapy. Also, additional studies of PRL and its regulation are needed in order to understand better the role of PRL in the physiology and pathophysiology of extrapituitary tissues, especially in the immune organs.

Keywords

Hyperprolactinemia, Metoclopramide, Spleen, Mice, Histology, CD4+/ CD8+ T cells, IHC

Status: Abstract Accepted (Oral Presentation)