| Repair and regeneration of damaged muscles using barium chloride and low-dose ionizing radiation in a rat model. |
| Paper ID : 1064-ISCHU |
| Authors |
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Esraa Ahmed Aboelfadl *1, Ola Hassan Elhabit1, Rasha Salah Elbeltagy1, Hesham Raffat Shawky1, Ahmed Esmat Abdel Moneim1, soheir saad korraa2 1Helwan University 2National Research Center for Radiation Research and Technology (NCCRT), Atomic Energy Authority |
| Abstract |
| There is growing evidence that, low dose ionizing radiation have bio-stimulatory effects. Several studies have revealed that low-dose ionizing radiation (IR) can be beneficial to living beings. It is deliberate that low dose ionizing radiation has an anti-inflammatory effect within the treatment of inflammation-related diseases. The aim of this study was to investigate the effect of low dose ionizing radiation on the damaged rat's skeletal muscle induced by barium chloride (BaCl2), determine serum levels of AST, ALT, Creatinine, LDH, assess the expression of genes that regulate skeletal muscle regeneration and differentiation MyoG and Myf5 as well as genes which is responsible for mitochondrial biogenesis Tfam and Nrf1, and examine the histological changes in muscle tissue. Adult male albino rats 2-3 months age, weighing 100-200 g. Animals were divided into 3 groups (9 rats/group) all were injected intramuscularly: Group 1 was injected with 50 µl of normal saline (0.9% NaCl) to the right tibialis anterior (TA) muscle. Group 2: injected with 50 µl of 1.2% BaCL2 into the right TA muscle. Group 3 was injected with 50 µl of 1.2% BaCL2 to the right TA muscle and at the next day the injected right leg was exposed to 0.5 Gy of γ-rays. After a week, the muscles of the rat were dissected for investigation and the results were discussed to heal the directly exposed ailing muscle. Results showed that the low dose of γ-radiation decreased the serum levels of AlT, AST, Creatinine and LDH activities which indicate diminution in muscle damage. Moreover, -radiation maintained the function of skeletal muscle fibers via increased the transcription factor MyoG and the regulatory myogenic factor Myf5. In addition, there was an increase in mitochondrial capacity by increasing Nrf1 and Tfam which defend mtDNA against ROS and degradation. The histopathological results of muscle tissues exposed directly to γ-radiation confirmed the above results. In conclusion, the current data may suggest the beneficial use of low dose ionizing radiation as a treatment tool in many muscular damage-related diseases. |
| Keywords |
| Muscular damage, Barium chloride, γ-radiation, Nrf1, Tfam, mtDNA |
| Status: Abstract Accepted (Poster Presentation) |