| Saline Lake-Derived Streptomyces thinghirensis WAE1: A Promising Source of Active Metabolites Agents |
| Paper ID : 1056-ISCHU |
| Authors |
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Mohamed E. Osman, Amany A. Abo Elnasr, Eslam Tarek * Botany and Microbiology Department, Faculty of Science, Helwan University, Ain Helwan, Cairo 11795, Egypt. |
| Abstract |
| This study aimed to assess the production of bioactive metabolites by Streptomyces thinghirensis WAE1, which is used as an indicator for activity against Streptococcus pneumoniae ATCC 49619. The study found that the highest level of biological activity was observed when glucose was the carbon source, soybean meal was used as the nitrogen source, a 6% seed inoculum was employed, the pH was set at 8, and the incubation period lasted for 8 days at a temperature of 30 °C. The optimal conditions were determined using the one-at-a-time strategy method, with major factors affecting the bioactive metabolites production through Plackett-Burman design (PB), Response surface methodology (RSM) with a central composite design (CCD), and temperature. The optimized media yielded an antibacterial activity of 44.23±0.33 mm against S. pneumoniae, which closely aligned with the predicted value of 43.12 mm. The optimized media showed a 74.92% increase in antibacterial production compared to the unoptimized medium. The selected approach demonstrated cost-effectiveness, efficacy, and potential applicability in identifying antibacterial drugs from a diverse range of metabolites produced by S. thinghirensis WAE1. |
| Keywords |
| Streptomyces thinghirensis, optimization, Plackett–Burman design, GC-mass |
| Status: Abstract Accepted (Poster Presentation) |